Simulation
EXPLANATION:
The simulation experiments offer information to the students that Rf value of the analyte increases and decreases with the rise and decrease of the polarity of the solvent system for the same stationary phase.
As the solvent system moves over the surface of the stationary phase, it transports the analyte over the particles of the stationary phase. If, a highly polar solvent is used, it will interact strongly with the molecules of stationary phase and will leave only few sites on the surface of stationary phase, free to bind with the analyte. The analyte will, therefore, move quickly over the stationary phase. Similarly, polar groups in the solvent can interact strongly with polar functionality in the analyte and prevent interaction of the analyses with the surface of the stationary phase. Among all the mobile phases tried, the separation of bands was found to be good in the solvent system Methanol: Ammonia (pH 6.8). So, further analysis was done by using the optimized mobile phase Methanol: Ammonia (pH 6.8).