Procedure

• A stock solution of standard cholesterol was prepared by dissolving cholesterol(0.05 mg/ml) in toluene and sonicated for 10 minutes over an ultrasonic bath.
  
• Edible oils were mixed with toluene and sonicated for 30 minutes for proper mixing.
  
• The solution was filtered through Whatman No. 41 filter paper and filtrate was used as sample solution.
• A 20cm × 10cm HPTLC plate coated with silica gel 60 F 254 and alumina60 F 254 (E. Merck, Darmstadt, Germany) was used for analysis.
• The samples were applied at 10 mm from the base of HPTLC plate by means of a Camag (Switzerland) Linomat V sample applicator using a syringe (100µL, Hamilton, Bonaduz, Switzerland).
• A linear calibration curve was obtained on applying the increasing concentration of standard amino acids in the range (200-1400 ng).
• HPTLC analysis was performed on a computerized densitometer scanner 3, controlled by WinCATS planar chromatography manager version 1.4.4. (CAMAG, Switzerland).
• Plate was developed to a distance of 80 mm, in a Camag twin-trough chamber with mobile phase [n-Hexane: Diethyl ether: MeOH:: 5:2:0.5 (v/v)].
• Plates were evaluated by densitometry at 200 nm with a Camag Scanner 3 for quantification.